Programme

STRUCTURE-FUNCTION RELATIONSHIPS OF PROTEINS. FROM AMINOACIDS to MYOGLOBIN AND HEMOGLOBIN.
STRUCTURAL AND FUNCTIONAL PROPERTIES OF THE ENZYMES. FROM THE MICHAELIS-MENTEN EQUATION TO THE PRE-STEADY-STATE AND THE INHIBITION MECHANISMS.
MOST IMPORTANT CYCLES AND METABOLIC PATHWAYS OF CARBS, LIPIDS AND PROTEINS.

Core Documentation

RECEPTION: MONDAY, FROM 3 TO 4 AM.

MCKEE, BIOCHIMICA, MCGRAW - HILL
NELSON, COX, I PRINCIPI DI BIOCHIMICA DI LEHININGER, ZANICHELLI
VOET, VOET, PRATT, FONDAMENTI DI BIOCHIMICA, ZANICHELLI
STRYER, BIOCHIMICA, ZANICHELLI
RAWN, BIOCHIMICA MCGRAW-HILL
MORAN ET AL., BIOCHIMICA MCGRAW-HILL
GARRET, GRISHAM, BIOCHIMICA, ZANICHELLI

Reference Bibliography

MCKEE, BIOCHIMICA, MCGRAW - HILL NELSON, COX, I PRINCIPI DI BIOCHIMICA DI LEHININGER, ZANICHELLI VOET, VOET, PRATT, FONDAMENTI DI BIOCHIMICA, ZANICHELLI STRYER, BIOCHIMICA, ZANICHELLI RAWN, BIOCHIMICA MCGRAW-HILL MORAN ET AL., BIOCHIMICA MCGRAW-HILL GARRET, GRISHAM, BIOCHIMICA, ZANICHELLI

Type of delivery of the course

THE TEACHING IS ARTICULATED IN CLASSROOM LESSONS AND PRACTICAL EXERCISES.

Attendance

THE FREQUENCY IS OBLIGATORY IN ORDER TO FACE THE WRITTEN AND ORAL TESTS IN ITINERE AND IN THE EXAMINATION.

Type of evaluation

DURING THE COURSE THERE ARE THREE EXAMINATIONS. THE OVERALL EXAMINATION CONSISTS OF A WRITTEN TEST AND AN ORAL COLLOQUIUM.

Programme

The practice module of the course of Biochemistry includes three laboratory experiences, each lasting 2 hours. The topics of the experiences are:
1. Separation of blood in its cellular and plasma component. Fractionation of blood proteins by saline and ethanol precipitation.
2. Quantification of plasma proteins by the Bradford method. Preparation of samples to be loaded on SDS-PAGE. Loading on polyacrylamide gel of fractionated proteins.
3. Characterization of the enzymatic activity of lactate dehydrogenase.

Core Documentation

MCKEE, BIOCHIMICA, MCGRAW - HILL. ALTRI TESTI: NELSON, COX, I PRINCIPI DI BIOCHIMICA DI LEHININGER, ZANICHELLI VOET, VOET, PRATT, FONDAMENTI DI BIOCHIMICA, ZANICHELLI STRYER, BIOCHIMICA, ZANICHELLI RAWN, BIOCHIMICA, MCGRAW-HILLMORAN ET AL., BIOCHIMICA, MCGRAW-HILL GARRET, GRISHAM, BIOCHIMICA, ZANICHELLI


RECEPTION TIME: Monday 10 am

Type of delivery of the course

The practice module of the Biochemistry course includes three laboratory experiences, each lasting 2 hours. The topics of the experiences are: 1. Separation of blood in its cellular and plasma component. Fractionation of blood proteins by saline and ethanol precipitation. 2. Quantification of plasma proteins by the Bradford method. Preparation of samples to be loaded on SDS-PAGE. Loading on polyacrylamide gel of fractionated proteins. 3. Characterization of the enzymatic activity of lactate dehydrogenase. The experimental data obtained during the practical experiences must be analyzed by the students using appropriate software (Excel). The results obtained will be discussed together with the teacher during a classroom lecture.

Type of evaluation

Participation at the three laboratory experiences is mandatory in order to pass the exam of Biochemistry. Therefore, the module I of the Biochemistry Course does not require a specific evaluation, but the active participation of the student in the activities carried out. In fact, the student is required to analyze the experimental data obtained in the three experiences through the use of appropriate software (Excel). The results obtained will then be analyzed and discussed together with the teacher during a classroom lecture.