20402369 - MICROBIAL GENETICS

The main educational goal of the genetics of microorganisms course is to provide students with knowledge on:
1) the prokaryotic genome and the mechanisms that ensure its replication, transcription, translation and integrity throughout generations;
2) the regulation mechanisms of the genetic expression in bacteria;
3) the mechanisms responsible for horizontal gene transfer (conjugation, transformation and transduction);
4) the molecular basis of cellular differentiation and adaptive response.
Students will also acquire basic knowledge on genetic engineering and omic approaches to study microbial communities.

IMPERI FRANCESCO

teacher profile | materiale didattico

Fruizione: 20401664 GENETICA DEI MICRORGANISMI in Biologia per la ricerca molecolare, cellulare e fisiopatologica LM-6 N0 IMPERI FRANCESCO

Programma

The course aims at providing to the student:
- adequate knowledge and understanding on advanced methodologies in bacterial genetics, appropriate methodologies and communication skills for the study of genotype-phenotype relationships in bacteria;
- adequate applied knowledge for the evaluation and drafting of experimental approaches, and for retrieving and critically reviewing relevant scientific literature from on-line databases (making judgements).

Programme
- structure, organization, functioning and evolution of the bacterial chromosome;
- methods for the sequencing and analysis of bacterial genomes;
- structure, organization, function and evolution of mobile genetic elements (plasmids and transposons);
- genetic and molecular bases of horizontal gene transfer;
- genetic and molecular bases of gene expression regulation and bistability in bacteria;
- genetic bases and experimental approaches for the study of antibiotic resistance and tolerance;
- methods for the identification and validation of essential genes;
- approaches for the identification and study of unculturable microorganisms


Testi Adottati

Selected topics will be available on suggested Bacterial Genetics books, as indicated in the following reference list. Specific research papers and experimental protocols will be provided during theoretic lessons on advanced research topics and laboratory practices.
Innovative teaching will be promoted by sharing power-point presentations illustrating the main topics of the course via email. Moreover, students will be encouraged to make use of web-based resources and databases to autonomously increase their knowledge on specific advanced topics.


Modalità Erogazione

The course is based on theoretical lessons in classroom and on laboratory practice.

Modalità Valutazione

The student will be asked to autonomously select a scientific publication to be presented at the beginning of the exam. Additional theoretical and practical concepts discussed during the lessons of the course will be objects of the exam. Particular attention will be given to the ability of the student to apply the knowledge acquired during the course to solve new problems in the biotechnological scenario. Final mark will be attribute as follow: - 50% understanding of and ability to present and discuss properly the scientific publication selected by the student; - 50% acquisition and understanding of knowledge; communication skills; Outstanding students will be certified with honors (lode).