20410255 - BIOTECNOLOGIE MOLECOLARI

The aim of the course is to provide methodologies and technologies knowledge of basic and applied advanced research in molecular biotechnologies.

CERVELLI MANUELA

teacher profile | materiale didattico

Mutuazione: 20410255 BIOTECNOLOGIE MOLECOLARI in Biologia per la ricerca molecolare, cellulare e fisiopatologica LM-6 CERVELLI MANUELA

Programma

1. BASIC TECHNOLOGIES OF GENE ANALYSIS; RESTRICTION ENZYMES; ELECTROPHORETIC ANALYSIS. 2. VECTORS; CLONING. 3. PCR; REAL TIME PCR; PCR APPLICATIONS. 4. GENETIC VARIABILITY APPLIED TO FORENSIC SCIENCE 5. GENE IDENTIFICATION; GENOMIC LIBRERIES AND cDNA LIBRERIES; YEAST TWO HYBRID SYSTEM 6. MUTAGENESIS. 7. RECOMBINANT PROTEIN PRODUCTION AND PURIFICATION. 8. FROM SANGER SEQUENCING TO NEXT-GENERATION SEQUENCING. 9. POST-GENOMICS ANALYSIS; TRANSCRIPTOMIC ANALYSIS; REPORTER GENES. 10. PROTEOMIC ANALYSIS. 11. PRODUCTION AND ANALYSIS OF TRANSGENIC PLANTS. 12. GENETIC MODIFICATION OF ANIMAL CELLS; TRANSGENIC ANIMALS CONSTRUCTION. 13. GENE TRAPPING; GENE TARGETING. 14. ANIMAL CLONING; GENE THERAPY. 15. THE CRISPR-CAS SYSTEM: EMERGING TECHNOLOGY AND APPLICATION AND APPLICATIONS.

Testi Adottati

Recommended textbooks:
1. ANALISI DEI GENI E GENOMI, REECE, EDISES.
2. DNA RICOMBINANTE, J.D.WATSON, A.A. CAUDY, R.M. MYERS E J.A. WITKOWSKI, ZANICHELLI.

Lecture slides will be provided.
The professor receives every day from 10.00 to 11.00 by appointment via e-mail: manuela.cervelli@uniroma3.it


Modalità Erogazione

The course will take place with lectures and training in didactic laboratories.

Modalità Valutazione

Oral examination. The test is in an interview of about 30 minutes during the period the student has to answer a series of questions (5-6) including most of the topics covered during the course. Each vote takes into account the acquired knowledge (50%), the student's ability to integrate different concepts and concepts and insert them correctly in contextual problems (30%), and the ability to offer a clear language, correct to the contents (20%).