This course will also give information on emerging technologies, such as transcriptomics, proteomics, metabolomics, and will describe their importance in plant biotechnology research.
Another important aim of the course is to prepare students to conduct laboratory work and research in the sectors of plant biotechnology and food industry, as well as to critically analyze scientific information.
TAVLADORAKI PARASKEVIteacher profile materiale didattico
Mutuazione: 20410303 BIOTECNOLOGIE PER IL MIGLIORAMENTO GENETICO DELLE PIANTE in Biologia per la ricerca molecolare, cellulare e fisiopatologica LM-6 TAVLADORAKI PARASKEVI
ProgrammaTHREE ARE THE MAIN ARGUMENTS OF THE COURSE: GENE TRANSFER TECHNIQUES; APPLICATIONS OF PLANT BIOTECHNOLOGY; ANALYSIS OF RISKS AND BENEFITS OF PLANT GENETIC MANIPULATION.
IN DETAIL, DURING THE COURSE THE FOLLOWING ARGUMENTS ARE DISCUSSED:
TECHNIQUES FOR GENE TRANSFER INTO THE NUCLEAR AND CHLOROPLASTIC GENOME; TECHNIQUES FOR IN VITRO CULTURE OF PLANT CELLS; USE OF SELECTABLE GENE MARKERS AND REPORTER GENES; STRATEGIES TO REMOVE SELECTABLE GENE MARKERS FROM TRANSGENIC PLANTS; INDUCIBLE EXPRESSION OF TRANSGENES IN PLANTS; TRANSIENT EXPRESSION OF GENES IN PLANTS; GENE SILENCING IN PLANTS; ‘GENE TARGETING’ IN PLANTS; GENOME EDITINGIN IN PLANTS; PLANTS WITH IMPROVED NUTRITIONAL VALUE; PRODUCTION OF ANTIBODIES AND VACCINES IN TRANSGENIC PLANTS; BIOTECHNOLOGICAL STRATEGIES TO OBTAIN PLANTS RESISTANT TO HERBICIDES, PATHOGENS AND ENVIRONMENTAL STRESSES; PLANTS WITH INCREASED PHOTOSYNTHETIC RATE; BIOSAFETY AND BIOETHICAL ISSUES RELATED TO PLANT BIOTECHNOLOGY; EMERGING TECHNOLOGIES AND PLANT BIOTECHNOLOGY.
Testi Adottati-Recommended textbooks:
1. BIOCHEMISTRY AND MOLECULAR BIOLOGY OF PLANTS (2000) BUCHANAN B.B, GRUISSEM W., JONES R.L., AMERICAN SOCIETY OF PLANT PHYSIOLOGISTS, ROCKVILLE, MARYLAND.
2. BIOLOGIA CELLULARE E BIOTECNOLOGIE VEGETALI (2011) COZZOLINO S., DI SANSEBASTIANO G.P., FORNI C., GENRE A., LANFRANCO L., MICCHELI A., PASQUA G., TRAINOTTI L., VALLETTA A.
3. HANDBOOK OF PLANT BIOYECHNOLOGY (2004) CHRISTOU P., KLEE H., HOBOKEN, (N.J.), WILEY.
4. PLANT BIOTECHNOLOGY AND TRANSGENIC PLANTS (2002) OKSMAN-CALDENTEY K.-M., BARZ W.H., NEW YORK, DEKER.
-Scientific articles published in international journals (pdf files will be provided).
-Lecture notes will be provided.
Modalità ErogazioneThe course will take place with lectures
Modalità FrequenzaLABORATORY ACTIVITIES: TRANSFORMATION OF ARABIDOPSIS PLANTS; ANALYSIS OF TRANSGENIC PLANTS BY PCR, RT-PCR, WESTERN BLOT AND ENZYME ACTIVITY ASSAYS; ANALYSIS OF REPORTER GENES IN TRANSGENIC PLANTS; ANALYSIS OF FOOD MATERIAL FOR THE PRESENCE OF GENETICALLY MODIFIED MATERIAL.
Modalità ValutazioneThe exam can be performed in two ways: 1. Three mid-term written tests with attribution of a score effective for the final vote. Each written test consists of a brief description of 2-4 topics held during the course. Maximum score is 30/30, whereas minimum score for a positive outcome is 18/30. It is necessary to the obtain at least the minimum score to all three written exams. Final score is the average value of the three partial scores. It is possible to improve the final score with an oral exam. 2. Oral exam from the whole program. Maximum score is 30/30, whereas minimum score for a positive outcome is 18/30. The knowledge of the topics and the capacity for critical analysis will be evaluated. The active participation in the lectures and the laboratory work will be considered positively for the final score. The active participation in the laboratory work will be considered positively for the final score.