Programme

THE COURSE CONCERNS THE GENERAL UNDERSTANDING OF THE MOLECULAR MECHANISMS WHICH REGULATE THE BIOLOGICAL PROCESSES OCCURRING WITHIN A CELL, WITH PARTICULAR EMPHASYS ON GENETIC STRUCTURE, ORGANIZATION AND EXPRESSION OF PROKARYOTIC AND EUKARYOTIC ORGANISMS. FURTHERMORE, THE SUBJECT TAKES INTO CONSIDERATION THE MOST UPDATED MOLECULAR BIOLOGY EXPERIMENTAL PROCEUDURES UTILIZED IN BASIC AND APPLIED RESEARCH. THE TEACHING PROGRAM IS ORGANIZED AS FOLLOWS: GENOME ANATOMY, TRANSCRIPTOMES AND PROTEOMES; GENOME ACCESSIBILITY; TRANSCRIPTION INITIATION COMPLEX; RNA SYNTHESES AND MATURATION; PROTEOME SYNTHESES AND MATURATION; GENOME SEQUENCING; GENOME REPLICATION; REPAIR SYSTEMS; GENETIC RECOMBINATION; GENETIC ENGINEERING AND DNA RECOMBINANT TECHNIQUES.

Core Documentation

REFERENCE TEXTBOOK: BIOLOGIA MOLECOLARE, AMALDI ET AL., TERZA EDIZIONE, CASA EDITRICE ZANICHELLI. FURTHER SUGGESTED TEXTBOOKS: 1) GENOMI, BROWN, QUARTA EDIZIONE, CASA EDITRICE EDISES; 2) IL GENE, LEWIN, TERZA EDIZIONE COMPATTA, ZANICHELLI.

Type of delivery of the course

Oral exam with an assessment apportioned on the institutional (6 CFU) and exercise (3 CFU).

Type of evaluation

Oral examination. The oral exam consists in an interview of about 10 minutes and will focus on the topics covered during the laboratory experience. The day of the examination a written report on the laboratory experience has to be presented and discussed, demonstrating to have understood the theoretical and practical principles of the techniques carried out during the laboratory experience. Furthermore, the ability to critically discuss the obtained results is required. The evaluation is 3 CFU.