20401820-1 - MODULO 1

THE GENERAL MICROBIOLOGY COURSE IS INTENDED TO PROVIDE A SOLID KNOWLEDGE BACKGROUND ON:
1) THE STRUCTURE AND FUNCTION OF THE PROKARYOTIC CELL; 2) THE MECHANISMS OF REPLICATION, EXCHANGE, RECOMBINATION AND EXPRESSION OF DNA IN BACTERIA; 3) THE NUTRITION, GROWTH AND METABOLISM OF BACTERIA; 4) THE STRUCTURAL, GENETIC, AND METABOLIC DIVERSITY IN BACTERIA; 5) THE ANTIMICROBIAL DRUGS AND THE CONTROL OF MICROBIAL GROWTH;6) THE CONCEPT OF BACTERIAL SPECIES AND THE EVOLUTION AND THE TAXONOMY OF BACTERIA; 7) THE BASIC PRINCIPLES OF MICROBIAL ECOLOGY; 8) THE PRINCIPLES OF MOLECULAR VIROLOGY OF BACTERIOPHAGES AND ANIMAL VIRUSES 9) THE INTERACTIONS BETWEEN BACTERIA, VIRUSES AND HIGHER ORGANISMS.
teacher profile | teaching materials

Programme

Laboratory practice:
- Gram staining
- Viable cell count by plating assays
- Determination of the minimum inhibitory concentration and minimum bactericidal concentration of antibiotics
- Antibiogram (Kirby-Bauer assays)


Core Documentation

Title: Brock. Biology of microorganisms
Authors: Madigan, Martinko
Editor: Pearson


Type of delivery of the course

The laboratory exercises are held in the teaching laboratories over four consecutive days of a week. At each exercise, students will be provided with an experimental protocol that will be also used for the analysis of the results and for homework.

Attendance

Attendance at laboratory exercises is not mandatory but strongly recommended.

Type of evaluation

The exam is aimed at verifying the level of knowledge and comprehension of the topics of the program and the reasoning skills developed by the student. The evaluation is expressed in thirtieths (minimum mark 18/30, maximum mark 30/30 cum laude). The exam consists of a written text (facultative) and an oral examination. The written test includes both open and closed questions on the topics covered during lectures and laboratory exercises. Open questions are evaluated for accuracy, completeness of content as well as synthesis and elaboration skills. If the mark of the written exam is at least 18/30, the oral exam will be mainly focused on the discussion about the text and the main mistakes made by the student. If the student does not pass or accept the mark of the written text, or if the student does not take the written text, the oral exam will cover all the topics of the course. Property of language, clarity of exposition, acquired knowledge and ability to make connections between the different topics are evaluated during the oral examination. Overall the exam aims to verify the achievement of the objectives in terms of knowledge and comprehension of the topics, as well as communication skills.

teacher profile | teaching materials

Programme

practical laboratory exercises:
1. introduction to animal cell cultures
2. hemagglutination test as a titration or serological identification test

Core Documentation

didactic material is provided during the exercises

Type of delivery of the course

Generally the two different practical exercises are conducted in the biological didactic laboratories on different days, dividing the students into groups compatible with the manual skills and management of the group and repeating each individual exercise several times based on the total number of students. Now due to the suspension of the frontal teaching from the beginning of March after the first three lectures to face the coronavirus epidemic, a site dedicated to the course on the MOODLE platform has been activated. Students are asked to refer to the information present there and to register on the platform.

Attendance

attendance of the course is strongly recommended

Type of evaluation

The evaluation of the work done in the exercises is carried out by administering a final questionnaire to evaluate understanding and by discussing the results and procedures performed by students during the activity.