20410501 - FUNDAMENTALS OF MICROSCOPY

Provide the theoretical foundations and the experimental practice of microscopic techniques with particular reference to optical, electronic and probe microscopy.

Only for the Curriculum of Matter Physics, the teaching can be inserted into the Study Planet in substitution of "Elements of General Relativity, Astrophysics and Cosmology" or to "Elements of Terrestrial and Environmental Physics"
teacher profile | teaching materials

Programme

Historical background of microscopy, concept of resolution and Rayleigh limit, overview of microscopy techniques and use in different research fields.

Fundamentals of optical microscopy, reflection microscopy, metallography, transmission microscopy, use of polarized light.

Operating principles of electron microscopy, SEM, TEM, EDX. Use of SEM: detection of secondary and backscattered electrons, capture and morphometric analysis of images.

Principles of operation and components of a probe scanning microscope, atomic force microscopy in contact, atomic force microscopy in non-contact. Secondary scanning techniques. Resolution and artifacts.

Introduction to 2D and 3D image analysis, improvement of image quality with and without kernel use, segmentation, binarization and quantitative image analysis with open-access software.


Core Documentation

notes provided by the professor based on the slides used during the lectures

Type of delivery of the course

Lecturing in the classroom with video-aided projection of slides and laboratory practical classes. The lab classes comprise "hands on " sessions on dedicated microscopes

Type of evaluation

final oral examination

teacher profile | teaching materials

Programme

Provide the theoretical foundations and the experimental practice of microscopic techniques with particular reference to optical, electronic and probe microscopy.

Core Documentation

handouts provided in class

Type of delivery of the course

Il corso prevede 32 ore di lezione frontale e 24 ore di laboratorio (Dott. Luca Persichetti). Lecturing in the classroom with video-aided projection of slides and laboratory practical classes. The lab classes comprise "hands on " sessions on dedicated microscopes

Type of evaluation

final oral examination