Knowledge and understanding of the following aspects of biochemistry: biochemical terminology;
molecular bases of biological systems;
structure-function relationships of biological macromolecules;
enzyme catalysis;
major metabolic pathways;
molecular aspects of drug action
molecular bases of biological systems;
structure-function relationships of biological macromolecules;
enzyme catalysis;
major metabolic pathways;
molecular aspects of drug action
teacher profile teaching materials
The practice module of the course of Biochemistry is MANDATORY and includes three laboratory experiences, each lasting 2 hours. The topics of the experiences are:
1. Protein quantification by Bradford method.
2. Separation of proteins by SDS-PAGE method and determination of their molecular weights by migration distance.
3. Characterization of the enzymatic activity of lactate dehydrogenase.
The results obtained will be analyzed and discussed together with the teacher during a frontal lesson in the classroom.
- VOET, VOET, PRATT, FONDAMENTI DI BIOCHIMICA, ZANICHELLI STRYER, BIOCHIMICA, ZANICHELLI
- MCGRAW-HILLMORAN ET AL., BIOCHIMICA, ZANICHELLI.
- M. K. Campbell, S. O. Farrell, O. M. McDougal "Biochimica", Ed. Edises
RECEPTION TIME: Monday 10 am
Fruizione: 20410221 BIOCHIMICA in Scienze biologiche L-13 R DI MASI ALESSANDRA, POLTICELLI FABIO, DE SIMONE GIOVANNA
Programme
The Bioenergetics and Metabolism module (Prof. Alessandra di Masi) will cover the following topics: bioenergetics and types of biochemical reactions, glycolysis, gluconeogenesis, pentose phosphate pathway, principles of metabolic regulation, citric acid cycle, fatty acid catabolism, amino acid oxidation and urea cycle, oxidative phosphorylation.The practice module of the course of Biochemistry is MANDATORY and includes three laboratory experiences, each lasting 2 hours. The topics of the experiences are:
1. Protein quantification by Bradford method.
2. Separation of proteins by SDS-PAGE method and determination of their molecular weights by migration distance.
3. Characterization of the enzymatic activity of lactate dehydrogenase.
The results obtained will be analyzed and discussed together with the teacher during a frontal lesson in the classroom.
Core Documentation
- NELSON, COX, I PRINCIPI DI BIOCHIMICA DI LEHININGER, ZANICHELLI- VOET, VOET, PRATT, FONDAMENTI DI BIOCHIMICA, ZANICHELLI STRYER, BIOCHIMICA, ZANICHELLI
- MCGRAW-HILLMORAN ET AL., BIOCHIMICA, ZANICHELLI.
- M. K. Campbell, S. O. Farrell, O. M. McDougal "Biochimica", Ed. Edises
RECEPTION TIME: Monday 10 am
Type of delivery of the course
The practice module of the Biochemistry course includes three laboratory experiences, each lasting 2 hours. The experimental data obtained during the practical experiences must be analyzed by the students using appropriate software (Excel). The results obtained will be discussed together with the teacher during a classroom lectures.Attendance
Attendance is mandatory for classroom lectures. Attendance at laboratory exercises is optional.Type of evaluation
Participation in the three laboratory experiences is mandatory in order to take the Biochemistry exam. At the end of the laboratory experiences attended (2 out of 3 mandatory), the student is required to analyze the experimental data obtained using appropriate software (Excel). The student will be required to write a short report reporting the data obtained in each experience attended.Fruizione: 20410221 BIOCHIMICA in Scienze biologiche L-13 R DI MASI ALESSANDRA, POLTICELLI FABIO, DE SIMONE GIOVANNA
teacher profile teaching materials
- Extraction of proteins from egg white and their quantification using the Bradford method (first experience)
- Preparation of samples for loading on SDS-PAGE (second experience)
- Characterisation of the enzymatic activity of lactate dehydrogenase (third experience)
Fruizione: 20410221 BIOCHIMICA in Scienze biologiche L-13 R DI MASI ALESSANDRA, POLTICELLI FABIO, DE SIMONE GIOVANNA
Programme
The practical module of the course includes three laboratory experiences. The topics of the experiences are:- Extraction of proteins from egg white and their quantification using the Bradford method (first experience)
- Preparation of samples for loading on SDS-PAGE (second experience)
- Characterisation of the enzymatic activity of lactate dehydrogenase (third experience)
Core Documentation
The slides of each lecture will be made availableType of delivery of the course
The laboratory exercises will all be carried out in presence.Attendance
MandatoryType of evaluation
The final grade will evaluate the following parameters: a) Appropriateness, correctness and congruence of the acquired knowledge and skills; b) Appropriate use of the specific language of the discipline; c) Expressive capacity; d) Logical skills; e) Ability to synthesize; f) Ability to link different topics. Consequently, the judgment may be: a) Sufficient (from 18 to 20/30) b) Fair (21 to 23) c) Good (from 24 to 26) d) Excellent (from 27 to 29) e) Excellent (30)