TO GET A GOOD COMMAND OF THE BIOCHEMICAL TERMINOLOGY; TO REACH A GOOD KNOWLEDGE OF THE MOLECULAR BASES OF THE BIOLOGICAL SYSTEM, OF THE BIOLOGICAL MACROMOLECULES STRUCTURE-FUNCTION RELATIONSHIPS, OF THE METABOLIC PATHWAYS, WITH PARTICULAR ATTENTION TO THEIR INTERACTIONS AND MOLECULAR COMPLEXITY.
teacher profile teaching materials
MCKEE, BIOCHIMICA, MCGRAW - HILL
NELSON, COX, I PRINCIPI DI BIOCHIMICA DI LEHININGER, ZANICHELLI
VOET, VOET, PRATT, FONDAMENTI DI BIOCHIMICA, ZANICHELLI
STRYER, BIOCHIMICA, ZANICHELLI
RAWN, BIOCHIMICA MCGRAW-HILL
MORAN ET AL., BIOCHIMICA MCGRAW-HILL
GARRET, GRISHAM, BIOCHIMICA, ZANICHELLI
Programme
THE PROGRAM CONSISTS IN THE STUDY STRUCTURAL AND FUNCTIONAL PROPERTIES OF THE ENZYMES. FROM THE MICHAELIS-MENTEN EQUATION TO THE PRE-STEADY-STATE AND THE INHIBITION MECHANISMS.Core Documentation
RECEPTION: MONDAY, FROM 10 TO 11 AM.MCKEE, BIOCHIMICA, MCGRAW - HILL
NELSON, COX, I PRINCIPI DI BIOCHIMICA DI LEHININGER, ZANICHELLI
VOET, VOET, PRATT, FONDAMENTI DI BIOCHIMICA, ZANICHELLI
STRYER, BIOCHIMICA, ZANICHELLI
RAWN, BIOCHIMICA MCGRAW-HILL
MORAN ET AL., BIOCHIMICA MCGRAW-HILL
GARRET, GRISHAM, BIOCHIMICA, ZANICHELLI
Type of delivery of the course
THE TEACHING IS ARTICULATED IN CLASSROOM LESSONS. THE FREQUENCY IS OBLIGATORY IN ORDER TO FACE THE WRITTEN AND ORAL TESTS IN ITINERE AND IN THE EXAMINATION.Type of evaluation
DURING THE COURSE THERE ARE THREE EXAMINATIONS. THE OVERALL EXAMINATION CONSISTS OF A WRITTEN TEST AND AN ORAL COLLOQUIUM. teacher profile teaching materials
1. Separation of blood in its cellular and plasma component. Fractionation of blood proteins by saline and ethanol precipitation.
2. Quantification of plasma proteins by the Bradford method. Preparation of samples to be loaded on SDS-PAGE. Loading on polyacrylamide gel of fractionated proteins.
3. Characterization of the enzymatic activity of lactate dehydrogenase.
RECEPTION TIME: Monday 10 am
Programme
The practice module of the course of Biochemistry includes three laboratory experiences, each lasting 2 hours. The topics of the experiences are:1. Separation of blood in its cellular and plasma component. Fractionation of blood proteins by saline and ethanol precipitation.
2. Quantification of plasma proteins by the Bradford method. Preparation of samples to be loaded on SDS-PAGE. Loading on polyacrylamide gel of fractionated proteins.
3. Characterization of the enzymatic activity of lactate dehydrogenase.
Core Documentation
MCKEE, BIOCHIMICA, MCGRAW - HILL. ALTRI TESTI: NELSON, COX, I PRINCIPI DI BIOCHIMICA DI LEHININGER, ZANICHELLI VOET, VOET, PRATT, FONDAMENTI DI BIOCHIMICA, ZANICHELLI STRYER, BIOCHIMICA, ZANICHELLI RAWN, BIOCHIMICA, MCGRAW-HILLMORAN ET AL., BIOCHIMICA, MCGRAW-HILL GARRET, GRISHAM, BIOCHIMICA, ZANICHELLIRECEPTION TIME: Monday 10 am
Type of delivery of the course
The practice module of the Biochemistry course includes three laboratory experiences, each lasting 2 hours. The topics of the experiences are: 1. Separation of blood in its cellular and plasma component. Fractionation of blood proteins by saline and ethanol precipitation. 2. Quantification of plasma proteins by the Bradford method. Preparation of samples to be loaded on SDS-PAGE. Loading on polyacrylamide gel of fractionated proteins. 3. Characterization of the enzymatic activity of lactate dehydrogenase. The experimental data obtained during the practical experiences must be analyzed by the students using appropriate software (Excel). The results obtained will be discussed together with the teacher during a classroom lecture.Type of evaluation
Participation to the three laboratory experiences is mandatory in order to pass the exam of Biochemistry. Therefore, the module I of the Biochemistry Course does not require a specific evaluation, but the active participation of the student in the activities carried out. In fact, the student is required to analyze the experimental data obtained in the three experiences through the use of appropriate software (Excel). The results obtained will then be analyzed and discussed together with the teacher during a classroom lecture.