Programme

LABORATORY INSTRUMENTS AND EQUIPMENT HANDLING (PLASTIC GLASSWARE, CHEMICAL HOOD, TISSUE CULTURE CABINET, INCUBATORS, BENCH CENTRIFUGES, THERMOSTATIC BATHS, WEIGHING BALANCES, PHMETERS, MAGNETIC AGITATORS, ELECTROPHORETIC APPARATUS); E. COLI CULTURING TECHNIQUES; CELL CULTURE MEDIA (DIFFERENT GROWTH MEDIA, BUFFERS; PH INDICATORS, ANTIBIOTICS, CHROMOGENS); BASIC DNA RECOMBINANT TECHNIQUES; BASIC PCR TECHNIQUES; E. COLI TRANSFORMATON TECHNIQUES; BASIC NUCLEIC ACID ELECTROPHORETIC TECHNIQUES; RESTRICTION ENZYMES BASIC TECHNIQUES; DNA SEQUENCING AND PROTEIN ANALYSIS.

Core Documentation

REFERENCE TEXT: BIOLOGIA MOLECOLARE, AMALDI ET AL., TERZA EDIZIONE, CASA EDITRICE ZANICHELLI.

Reference Bibliography

REFERENCE TEXT: BIOLOGIA MOLECOLARE, AMALDI ET AL., TERZA EDIZIONE, CASA EDITRICE ZANICHELLI.

Type of delivery of the course

The course will take place with lectures and training in didactic laboratories.

Attendance

The course will take place with lectures and training in didactic laboratories.

Type of evaluation

Oral examination. The oral exam consists in an interview of about 10 minutes and will focus on the topics covered during the laboratory experience. The vote takes into account the knowledge acquired (50%), the student's ability to integrate different notions and concepts and to insert them correctly in contextual situations (30%), and the ability to offer a clear language, correct and adequate to the contents ( 20%).

Programme

THE COURSE OF MOLECULAR BIOLOGY IS PART OF THE TEACHING ACTIVITIES OF THE DEGREE COURSE IN LIFE SCIENCES. THE COURSE THROUGH FRONTAL LESSONS AND LABORATORY TRAINING AIMS TO PROVIDE THE GENERAL UNDERSTANDING OF THE MOLECULAR MECHANISMS WHICH REGULATE THE BIOLOGICAL PROCESSES OCCURRING WITHIN A CELL, WITH PARTICULAR EMPHASYS ON GENETIC STRUCTURE, ORGANIZATION AND EXPRESSION OF PROKARYOTIC AND EUKARYOTIC ORGANISMS. TOOLS WILL BE GIVEN TO UNDERSTAND THE MAIN MOLECULAR BIOLOGY STRATEGIES AND TECHNIQUES UTILIZED TO STUDY GENES AND GENOMES. FURTHERMORE, THE SUBJECT TAKES INTO CONSIDERATION THE MOST UPDATED MOLECULAR BIOLOGY EXPERIMENTAL PROCEDURES UTILIZED IN BASIC AND APPLIED RESEARCH. THE STUDENTS WILL GET ACQUAINTED WITH THEORETICAL KNOWLEDGES AND METHODOLOGICAL SKILLS THAT WILL ENABLE THEM TO APPLY THE CORRECT STUDY TO SOLVE SPECIFIC PROBLEMS IN THE FIELD OF MOLECULAR BIOLOGY.
THE COURSE CONCERNS THE GENERAL UNDERSTANDING OF THE MOLECULAR MECHANISMS WHICH REGULATE THE BIOLOGICAL PROCESSES OCCURRING WITHIN A CELL, WITH PARTICULAR EMPHASYS ON GENETIC STRUCTURE, ORGANIZATION AND EXPRESSION OF PROKARYOTIC AND EUKARYOTIC ORGANISMS. FURTHERMORE, THE SUBJECT TAKES INTO CONSIDERATION THE MOST UPDATED MOLECULAR BIOLOGY EXPERIMENTAL PROCEUDURES UTILIZED IN BASIC AND APPLIED RESEARCH. THE TEACHING PROGRAM IS ORGANIZED AS FOLLOWS: GENOME ANATOMY, TRANSCRIPTOMES AND PROTEOMES; GENOME ACCESSIBILITY; TRANSCRIPTION INITIATION COMPLEX; RNA SYNTHESES AND MATURATION; PROTEOME SYNTHESES AND MATURATION; GENOME SEQUENCING; GENOME REPLICATION; DNA REPAIR SYSTEMS; GENETIC RECOMBINATION; GENETIC ENGINEERING AND DNA RECOMBINANT TECHNIQUES.

Core Documentation

BIOLOGIA MOLECOLARE, AMALDI ET AL., TERZA EDIZIONE, CASA EDITRICE ZANICHELLI

The professor receives every day from 8.00 to 9.00 by appointment via e-mail: giovanni.antonini@uniroma3.it

Type of delivery of the course

Attendance is strongly recommended

Type of evaluation

The exam will consist of an oral test on the topics presented in the program